Can a teacher-led mindfulness intervention for new school entrants improve child outcomes? Protocol for a school cluster randomised controlled trial.

The first years of school are critical in establishing a foundation for positive long-term academic, social and well-being outcomes. Mindfulness-based interventions may help students transition well into school, but few robust studies have been conducted in this age group.

We aim to determine whether compared with controls, children who receive a mindfulness intervention within the first years of primary school have better: (1) immediate attention/short-term memory at 18 months post-randomisation (primary outcome); (2) inhibition, working memory and cognitive flexibility at 18 months post-randomisation; (3) socio-emotional well-being, emotion-regulation and mental health-related behaviours at 6 and 18 months post-randomisation; (4) sustained changes in teacher practice and classroom interactions at 18 months post-randomisation.

Furthermore, we aim to determine whether the implementation predicts the efficacy of the intervention, and the cost effectiveness relative to outcomes.This cluster randomised controlled trial will be conducted in 22 primary schools in disadvantaged areas of Melbourne, Australia. 826 students in the first year of primary school will be recruited to detect between groups differences of Cohen’s d=0.25 at the 18-month follow-up.

Parent, teacher and child-assessment measures of child attention, emotion-regulation, executive functioning, socio-emotional well-being, mental health-related behaviour and learning, parent mental well-being, teacher well-being will be collected 6 and 18 months post-randomisation.

NOP56 Ribonucleoprotein (NOP56) Antibody
abx235795-100ug	Abbexa	100 ug	EUR 509
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Nop56 Ribonucleoprotein Homolog (Yeast) (NOP56) Antibody
20-abx114153	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul
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Human NOP56 Ribonucleoprotein (NOP56) ELISA Kit
abx381849-96tests	Abbexa	96 tests	EUR 911
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NOP56 siRNA
20-abx926151	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
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NOP56 siRNA
20-abx926152	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
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NOP56 antibody
70R-18923	Fitzgerald	50 ul	EUR 435
Description: Rabbit polyclonal NOP56 antibody

NOP56 Antibody
1-CSB-PA384993	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Form: Liquid Buffer: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Description: A polyclonal antibody against NOP56. Recognizes NOP56 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1:500-2000, ELISA:1:10000-20000

NOP56 Antibody
1-CSB-PA015939GA01HU	Cusabio	EUR 597.00 EUR 333.00	150ul 50ul
Form: Liquid Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20℃, Avoid freeze / thaw cycles. Antigen Affinity purified
Description: A polyclonal antibody against NOP56. Recognizes NOP56 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

NOP56 Polyclonal Antibody
ES8503-100ul	ELK Biotech	100ul	EUR 279
Description: A Rabbit Polyclonal antibody against NOP56 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

NOP56 Polyclonal Antibody
ES8503-50ul	ELK Biotech	50ul	EUR 207
Description: A Rabbit Polyclonal antibody against NOP56 from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, IHC, WB, ELISA

anti- NOP56 antibody
FNab05795	FN Test	100µg	EUR 548.75
Immunogen: NOP56 ribonucleoprotein homolog(yeast) Uniprot ID: O00567 Gene ID: 10528 Research Area: Neuroscience, Metabolism, Epigenetics
Description: Antibody raised against NOP56

NOP56 Polyclonal Antibody
ABP57510-003ml	Abbkine	0.03ml	EUR 158
Immunogen information: Synthesized peptide derived from NOP56 at AA range: 191-240 Applications tips:
Description: A polyclonal antibody for detection of NOP56 from Human, Mouse, Rat. This NOP56 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from NOP56 at AA range: 191-240

NOP56 Polyclonal Antibody
ABP57510-01ml	Abbkine	0.1ml	EUR 289
Immunogen information: Synthesized peptide derived from NOP56 at AA range: 191-240 Applications tips:
Description: A polyclonal antibody for detection of NOP56 from Human, Mouse, Rat. This NOP56 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from NOP56 at AA range: 191-240

NOP56 Polyclonal Antibody
ABP57510-02ml	Abbkine	0.2ml	EUR 414
Immunogen information: Synthesized peptide derived from NOP56 at AA range: 191-240 Applications tips:
Description: A polyclonal antibody for detection of NOP56 from Human, Mouse, Rat. This NOP56 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from NOP56 at AA range: 191-240

Anti-NOP56 Antibody
A04335	BosterBio	100ul	EUR 397
Description: Rabbit Polyclonal Antibody for NOP56 Antibody (NOP56) detection.tested for IHC, WB in Human, Mouse, Rat.

NOP56 Rabbit pAb
A8040-100ul	Abclonal	100 ul	EUR 384

NOP56 Rabbit pAb
A8040-200ul	Abclonal	200 ul	Ask for price

NOP56 Rabbit pAb
A8040-20ul	Abclonal	20 ul	Ask for price

NOP56 Rabbit pAb
A8040-50ul	Abclonal	50 ul	EUR 265

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46736-100ul	SAB	100ul	EUR 252

NOP56 Polyclonal Antibody
46736-50ul	SAB	50ul	EUR 187

Anti-NOP56 antibody
PAab05795	Lifescience Market	100 ug	EUR 386

Anti-NOP56 antibody
STJ98616	St John's Laboratory	200 µl	EUR 197
Description: Rabbit polyclonal to NOP56.

Anti-NOP56 antibody
STJ110344	St John's Laboratory	100 µl	EUR 393
Description: Nop56p is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by this gene is similar in sequence to Nop56p and is also found in the nucleolus. Expansion of a GGCCTG repeat from 3-8 copies to 1500-2500 copies in an intron of this gene results in spinocerebellar ataxia 36. Multiple transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of most of them has not been determined.

Mouse NOP56 shRNA Plasmid
20-abx975991	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
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NOP56 ELISA KIT|Human
EF001286	Lifescience Market	96 Tests	EUR 689

Human NOP56 shRNA Plasmid
20-abx957155	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
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NOP56 Recombinant Protein (Human)
RP021469	ABM	100 ug	Ask for price

NOP56 Recombinant Protein (Rat)
RP214226	ABM	100 ug	Ask for price

NOP56 Recombinant Protein (Mouse)
RP154574	ABM	100 ug	Ask for price

NOP56 ORF Vector (Human) (pORF)
ORF007157	ABM	1.0 ug DNA	EUR 95

Nop56 ORF Vector (Rat) (pORF)
ORF071410	ABM	1.0 ug DNA	EUR 506

Nop56 ORF Vector (Mouse) (pORF)
ORF051526	ABM	1.0 ug DNA	EUR 506

NOP56 sgRNA CRISPR Lentivector set (Human)
K1440801	ABM	3 x 1.0 ug	EUR 339

Nop56 sgRNA CRISPR Lentivector set (Mouse)
K4919601	ABM	3 x 1.0 ug	EUR 339

Nop56 sgRNA CRISPR Lentivector set (Rat)
K7169201	ABM	3 x 1.0 ug	EUR 339

Bovine Nucleolar protein 56, NOP56 ELISA KIT
ELI-16702b	Lifescience Market	96 Tests	EUR 928

Human Nucleolar protein 56, NOP56 ELISA KIT
ELI-35313h	Lifescience Market	96 Tests	EUR 824

Mouse Nucleolar protein 56, Nop56 ELISA KIT
ELI-46118m	Lifescience Market	96 Tests	EUR 865

NOP56 sgRNA CRISPR Lentivector (Human) (Target 1)
K1440802	ABM	1.0 ug DNA	EUR 154

NOP56 sgRNA CRISPR Lentivector (Human) (Target 2)
K1440803	ABM	1.0 ug DNA	EUR 154

NOP56 sgRNA CRISPR Lentivector (Human) (Target 3)
K1440804	ABM	1.0 ug DNA	EUR 154

Nop56 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K4919602	ABM	1.0 ug DNA	EUR 154

Nop56 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K4919603	ABM	1.0 ug DNA	EUR 154

Nop56 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K4919604	ABM	1.0 ug DNA	EUR 154

Nop56 sgRNA CRISPR Lentivector (Rat) (Target 1)
K7169202	ABM	1.0 ug DNA	EUR 154

Nop56 sgRNA CRISPR Lentivector (Rat) (Target 2)
K7169203	ABM	1.0 ug DNA	EUR 154

Nop56 sgRNA CRISPR Lentivector (Rat) (Target 3)
K7169204	ABM	1.0 ug DNA	EUR 154

NOP56 Protein Vector (Human) (pPB-C-His)
PV028625	ABM	500 ng	EUR 329

NOP56 Protein Vector (Human) (pPB-N-His)
PV028626	ABM	500 ng	EUR 329

NOP56 Protein Vector (Human) (pPM-C-HA)
PV028627	ABM	500 ng	EUR 329

NOP56 Protein Vector (Human) (pPM-C-His)
PV028628	ABM	500 ng	EUR 329

NOP56 Protein Vector (Mouse) (pPB-C-His)
PV206102	ABM	500 ng	EUR 603

NOP56 Protein Vector (Mouse) (pPB-N-His)
PV206103	ABM	500 ng	EUR 603

NOP56 Protein Vector (Mouse) (pPM-C-HA)
PV206104	ABM	500 ng	EUR 603

NOP56 Protein Vector (Mouse) (pPM-C-His)
PV206105	ABM	500 ng	EUR 603

NOP56 Protein Vector (Rat) (pPB-C-His)
PV285638	ABM	500 ng	EUR 603

NOP56 Protein Vector (Rat) (pPB-N-His)
PV285639	ABM	500 ng	EUR 603

NOP56 Protein Vector (Rat) (pPM-C-HA)
PV285640	ABM	500 ng	EUR 603

NOP56 Protein Vector (Rat) (pPM-C-His)
PV285641	ABM	500 ng	EUR 603

Nop56 3'UTR GFP Stable Cell Line
TU164211	ABM	1.0 ml	Ask for price

NOP56 3'UTR Luciferase Stable Cell Line
TU015823	ABM	1.0 ml	EUR 1394

Nop56 3'UTR Luciferase Stable Cell Line
TU114211	ABM	1.0 ml	Ask for price

NOP56 3'UTR GFP Stable Cell Line
TU065823	ABM	1.0 ml	EUR 1394

Nop56 3'UTR GFP Stable Cell Line
TU264084	ABM	1.0 ml	Ask for price

Nop56 3'UTR Luciferase Stable Cell Line
TU214084	ABM	1.0 ml	Ask for price

NOP56 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV628063	ABM	1.0 ug DNA	EUR 682

NOP56 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV628067	ABM	1.0 ug DNA	EUR 682

NOP56 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV628068	ABM	1.0 ug DNA	EUR 682

NOP56 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV792199	ABM	1.0 ug DNA	EUR 316

NOP56 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV792200	ABM	1.0 ug DNA	EUR 316

NOP56 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K1440805	ABM	3 x 1.0 ug	EUR 376

Nop56 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K4919605	ABM	3 x 1.0 ug	EUR 376

Nop56 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K7169205	ABM	3 x 1.0 ug	EUR 376

NOP56 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K1440806	ABM	1.0 ug DNA	EUR 167

NOP56 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K1440807	ABM	1.0 ug DNA	EUR 167

NOP56 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K1440808	ABM	1.0 ug DNA	EUR 167

Nop56 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K4919606	ABM	1.0 ug DNA	EUR 167

Nop56 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K4919607	ABM	1.0 ug DNA	EUR 167

Nop56 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K4919608	ABM	1.0 ug DNA	EUR 167

NOP56 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV628064	ABM	1.0 ug DNA	EUR 682

NOP56 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV628065	ABM	1.0 ug DNA	EUR 740

NOP56 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV628066	ABM	1.0 ug DNA	EUR 740

Nop56 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)
K7169206	ABM	1.0 ug DNA	EUR 167

Nop56 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)
K7169207	ABM	1.0 ug DNA	EUR 167

Nop56 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)
K7169208	ABM	1.0 ug DNA	EUR 167

Implementation factors will be measured throughout the study. Intention-to-treat analyses, accounting for clustering within schools and classes, will adopt a two-level random effects linear regression model to examine outcomes for the intervention versus control students. Unadjusted and analyses adjusted for baseline scores, baseline age, gender and family socioeconomic status will be conducted.

Ethics approval has been received by the Human Research Ethics Committee at the University of Melbourne. Findings will be reported in peer-review publications, national and international conference presentations and research snapshots directly provided to participating schools and families.