Consensus on the exercise and dosage variables of an exercise training programme for chronic non-specific neck pain: protocol for an international e-Delphi study.

Clinical guidelines and systematic reviews recommend exercise in the management of chronic non-specific neck pain. Although exercise training programmes that consist of both motor control exercise and exercises for the superficial cervical muscles (segmental exercises) are effective, the exercise variables including dosage vary considerably across trials or are poorly reported.

This study aims to gain expert consensus on these exercise variables so that they can be described clearly using intervention reporting checklists to inform clinical practice and future clinical trials.This protocol for an international Delphi study is informed by the Guidance on Conducting and REporting DElphi Studies recommendations and published to ensure quality, rigour and transparency.

The study will consist of three rounds using anonymous online questionnaires. Expert exercise professionals (physiotherapists, strength and conditioning coaches and so on) and academics in neck pain management will be identified through literature searches, peer referral and social media calls for expression of interest. In round 1, participants will answer open-ended questions informed by intervention and exercise reporting checklists. Responses will be analysed thematically by two independent reviewers.

In round 2, participants will rate their level of agreement with statements generated from round 1 and previous clinical trials using a 5-point Likert scale where 1=strongly disagree and 5=strongly agree. In round 3, participants will re-rate their agreement with statements that achieved consensus in round 2. Statements reaching consensus among participants must meet progressively increased a priori criteria at rounds 2 and 3, measured using descriptive statistics: median, IQR and percentage agreement.

Nop58/ Rat Nop58 ELISA Kit
ELI-44674r	Lifescience Market	96 Tests	EUR 886

Nop58 Ribonucleoprotein Homolog (Yeast) (NOP58) Antibody
20-abx114154	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul
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Human NOP58 Ribonucleoprotein (NOP58) ELISA Kit
abx381850-96tests	Abbexa	96 tests	EUR 911
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NOP58 antibody
70R-18924	Fitzgerald	50 ul	EUR 435
Description: Rabbit polyclonal NOP58 antibody

NOP58 Antibody
1-CSB-PA896703LA01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
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NOP58 Antibody
DF13192	Affbiotech	200ul	EUR 304
Description: NOP58 Antibody detects endogenous levels of NOP58.

NOP58 Antibody
1-CSB-PA015940GA01HU	Cusabio	EUR 597.00 EUR 333.00	150ul 50ul
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Description: A polyclonal antibody against NOP58. Recognizes NOP58 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

NOP58 siRNA
20-abx903599	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol
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NOP58 siRNA
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30604-100ul	SAB	100ul	EUR 252

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30604-50ul	SAB	50ul	EUR 187

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28438-100ul	SAB	100ul	EUR 252

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28438-50ul	SAB	50ul	EUR 187

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A14129-100ul	Abclonal	100 ul	EUR 308

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A14129-200ul	Abclonal	200 ul	EUR 459

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A14129-20ul	Abclonal	20 ul	EUR 183

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A14129-50ul	Abclonal	50 ul	EUR 223

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DF13192-BP	Affbiotech	1mg	EUR 195

NOP5/NOP58 antibody
70R-3412	Fitzgerald	50 ug	EUR 467
Description: Rabbit polyclonal NOP5/NOP58 antibody raised against the middle region of NOP5/NOP58

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A54768	EpiGentek	100 µg	EUR 570.55
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NOP58 Rabbit pAb
A4749-100ul	Abclonal	100 ul	EUR 308

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A4749-200ul	Abclonal	200 ul	EUR 459

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A4749-20ul	Abclonal	20 ul	EUR 183

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A4749-50ul	Abclonal	50 ul	EUR 223

anti- NOP58 antibody
FNab05796	FN Test	100µg	EUR 505.25
Recommended dilution: WB: 1:500-1:2000 IP: 1:500-1:1000 Immunogen: NOP58 ribonucleoprotein homolog(yeast) Uniprot ID: Q9Y2X3 Gene ID: 51602 Research Area: Metabolism, Developmental biology
Description: Antibody raised against NOP58

Anti-NOP58 antibody
PAab05796	Lifescience Market	100 ug	EUR 355

pDONR223-NOP58 Plasmid
PVTB01144-1	Lifescience Market	2 ug	EUR 356

Anti-NOP58 antibody
STJ24780	St John's Laboratory	100 µl	EUR 277
Description: The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs.

Anti-NOP58 antibody
STJ116064	St John's Laboratory	100 µl	EUR 277
Description: The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs.

NOP5/NOP58 Blocking Peptide
33R-5398	Fitzgerald	100 ug	EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of NOP5/NOP58 antibody, catalog no. 70R-3412

NOP58 Antibody, HRP conjugated
1-CSB-PA896703LB01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against NOP58. Recognizes NOP58 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

NOP58 Antibody, FITC conjugated
1-CSB-PA896703LC01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
Form: Liquid Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 >95%, Protein G purified
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NOP58 Antibody, Biotin conjugated
1-CSB-PA896703LD01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
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EF001287	Lifescience Market	96 Tests	EUR 689

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RP214229	ABM	100 ug	Ask for price

Human Nucleolar protein 58 (NOP58)
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MW: 75.6 kDa Buffer composition: Tris-based buffer with 50% glycerol.
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Nop58 ORF Vector (Rat) (pORF)
ORF071411	ABM	1.0 ug DNA	EUR 506

NOP58 ORF Vector (Human) (pORF)
ORF007158	ABM	1.0 ug DNA	EUR 95

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ORF051527	ABM	1.0 ug DNA	EUR 506

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K4828601	ABM	3 x 1.0 ug	EUR 339

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K6841801	ABM	3 x 1.0 ug	EUR 339

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Nop58 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K4828602	ABM	1.0 ug DNA	EUR 154

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K4828603	ABM	1.0 ug DNA	EUR 154

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K6841802	ABM	1.0 ug DNA	EUR 154

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K6841803	ABM	1.0 ug DNA	EUR 154

Nop58 sgRNA CRISPR Lentivector (Rat) (Target 3)
K6841804	ABM	1.0 ug DNA	EUR 154

NOP58 sgRNA CRISPR Lentivector (Human) (Target 1)
K1441002	ABM	1.0 ug DNA	EUR 154

NOP58 sgRNA CRISPR Lentivector (Human) (Target 2)
K1441003	ABM	1.0 ug DNA	EUR 154

NOP58 sgRNA CRISPR Lentivector (Human) (Target 3)
K1441004	ABM	1.0 ug DNA	EUR 154

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PV206106	ABM	500 ng	EUR 603

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NOP58 Protein Vector (Rat) (pPM-C-HA)
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PV285645	ABM	500 ng	EUR 603

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PV028629	ABM	500 ng	EUR 329

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PV028631	ABM	500 ng	EUR 329

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PV028632	ABM	500 ng	EUR 329

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QY-E03190	Qayee Biotechnology	96T	EUR 394

Nop58 3'UTR Luciferase Stable Cell Line
TU114212	ABM	1.0 ml	Ask for price

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TU164212	ABM	1.0 ml	Ask for price

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TU065825	ABM	1.0 ml	EUR 1521

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TU015825	ABM	1.0 ml	EUR 1521

NOP58 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV692263	ABM	1.0 ug DNA	EUR 682

NOP58 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV692267	ABM	1.0 ug DNA	EUR 682

NOP58 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV692268	ABM	1.0 ug DNA	EUR 682

Recombinant Candida Albicans NOP58 Protein (aa 1-516)
VAng-Lsx05762-inquire	Creative Biolabs	inquire	Ask for price
Description: Candida Albicans (strain SC5314 / ATCC MYA-2876) Nucleolar protein 58, recombinant protein.

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K4828605	ABM	3 x 1.0 ug	EUR 376

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K6841805	ABM	3 x 1.0 ug	EUR 376

NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K1441005	ABM	3 x 1.0 ug	EUR 376

NOP58 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)
LV692264	ABM	1.0 ug DNA	EUR 682

NOP58 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV692265	ABM	1.0 ug DNA	EUR 740

NOP58 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV692266	ABM	1.0 ug DNA	EUR 740

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K4828606	ABM	1.0 ug DNA	EUR 167

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K4828607	ABM	1.0 ug DNA	EUR 167

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)
K4828608	ABM	1.0 ug DNA	EUR 167

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)
K6841806	ABM	1.0 ug DNA	EUR 167

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)
K6841807	ABM	1.0 ug DNA	EUR 167

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)
K6841808	ABM	1.0 ug DNA	EUR 167

NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K1441006	ABM	1.0 ug DNA	EUR 167

NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K1441007	ABM	1.0 ug DNA	EUR 167

NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K1441008	ABM	1.0 ug DNA	EUR 167

Inferential statistics will be used to evaluate measures of agreement between participants (Kendall’s coefficient of concordance) and stability between rounds (Wilcoxon rank-sum test). Statements achieving consensus in round 3 will provide expert recommendations of the key exercise and dosage variables in the management of chronic non-specific neck pain.Ethical approval was provided by the University of Birmingham Ethics Committee (Ref:ERN_19-1857). Results will be disseminated through peer-reviewed publications and conference presentations.