Consensus on the exercise and dosage variables of an exercise training programme for chronic non-specific neck pain: protocol for an international e-Delphi study.

Clinical guidelines and systematic reviews recommend exercise in the management of chronic non-specific neck pain. Although exercise training programmes that consist of both motor control exercise and exercises for the superficial cervical muscles (segmental exercises) are effective, the exercise variables including dosage vary considerably across trials or are poorly reported.

This study aims to gain expert consensus on these exercise variables so that they can be described clearly using intervention reporting checklists to inform clinical practice and future clinical trials.This protocol for an international Delphi study is informed by the Guidance on Conducting and REporting DElphi Studies recommendations and published to ensure quality, rigour and transparency.

The study will consist of three rounds using anonymous online questionnaires. Expert exercise professionals (physiotherapists, strength and conditioning coaches and so on) and academics in neck pain management will be identified through literature searches, peer referral and social media calls for expression of interest. In round 1, participants will answer open-ended questions informed by intervention and exercise reporting checklists. Responses will be analysed thematically by two independent reviewers.

In round 2, participants will rate their level of agreement with statements generated from round 1 and previous clinical trials using a 5-point Likert scale where 1=strongly disagree and 5=strongly agree. In round 3, participants will re-rate their agreement with statements that achieved consensus in round 2. Statements reaching consensus among participants must meet progressively increased a priori criteria at rounds 2 and 3, measured using descriptive statistics: median, IQR and percentage agreement.

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ELI-44674r	Lifescience Market	96 Tests	EUR 886

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NOP58 siRNA
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70R-18924	Fitzgerald	50 ul	EUR 435
Description: Rabbit polyclonal NOP58 antibody

NOP58 Antibody
1-CSB-PA896703LA01HU	Cusabio	EUR 317.00 EUR 335.00	100ug 50ug
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DF13192	Affbiotech	200ul	EUR 304
Description: NOP58 Antibody detects endogenous levels of NOP58.

NOP58 Antibody
1-CSB-PA015940GA01HU	Cusabio	EUR 597.00 EUR 333.00	150ul 50ul
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anti- NOP58 antibody
FNab05796	FN Test	100µg	EUR 505.25
Recommended dilution: WB: 1:500-1:2000 IP: 1:500-1:1000 Immunogen: NOP58 ribonucleoprotein homolog(yeast) Uniprot ID: Q9Y2X3 Gene ID: 51602 Research Area: Metabolism, Developmental biology
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70R-3412	Fitzgerald	50 ug	EUR 467
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A14129-100ul	Abclonal	100 ul	EUR 308

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A14129-200ul	Abclonal	200 ul	EUR 459

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30604-100ul	SAB	100ul	EUR 252

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30604-50ul	SAB	50ul	EUR 187

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28438-100ul	SAB	100ul	EUR 252

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28438-50ul	SAB	50ul	EUR 187

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PAab05796	Lifescience Market	100 ug	EUR 355

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STJ24780	St John's Laboratory	100 µl	EUR 277
Description: The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs.

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STJ116064	St John's Laboratory	100 µl	EUR 277
Description: The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs.

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C30604	SAB	100ul	EUR 397

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20-abx974581	Abbexa	EUR 801.00 EUR 1121.00	150 µg 300 µg
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NOP5/NOP58 Blocking Peptide
33R-5398	Fitzgerald	100 ug	EUR 180
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NOP58 Antibody, HRP conjugated
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Human Nucleolar protein 58 (NOP58)
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ORF007158	ABM	1.0 ug DNA	EUR 95

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ORF071411	ABM	1.0 ug DNA	EUR 506

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ORF051527	ABM	1.0 ug DNA	EUR 506

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K1441001	ABM	3 x 1.0 ug	EUR 339

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K4828601	ABM	3 x 1.0 ug	EUR 339

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K1441002	ABM	1.0 ug DNA	EUR 154

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K1441003	ABM	1.0 ug DNA	EUR 154

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K6841802	ABM	1.0 ug DNA	EUR 154

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NOP58 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV692268	ABM	1.0 ug DNA	EUR 682

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VAng-Lsx05762-inquire	Creative Biolabs	inquire	Ask for price
Description: Candida Albicans (strain SC5314 / ATCC MYA-2876) Nucleolar protein 58, recombinant protein.

NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)
K1441005	ABM	3 x 1.0 ug	EUR 376

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)
K4828605	ABM	3 x 1.0 ug	EUR 376

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)
K6841805	ABM	3 x 1.0 ug	EUR 376

NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)
K1441006	ABM	1.0 ug DNA	EUR 167

NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)
K1441007	ABM	1.0 ug DNA	EUR 167

NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)
K1441008	ABM	1.0 ug DNA	EUR 167

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)
K4828606	ABM	1.0 ug DNA	EUR 167

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)
K4828607	ABM	1.0 ug DNA	EUR 167

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K4828608	ABM	1.0 ug DNA	EUR 167

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NOP58 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)
LV692265	ABM	1.0 ug DNA	EUR 740

NOP58 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
LV692266	ABM	1.0 ug DNA	EUR 740

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)
K6841806	ABM	1.0 ug DNA	EUR 167

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)
K6841807	ABM	1.0 ug DNA	EUR 167

Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)
K6841808	ABM	1.0 ug DNA	EUR 167

Inferential statistics will be used to evaluate measures of agreement between participants (Kendall’s coefficient of concordance) and stability between rounds (Wilcoxon rank-sum test). Statements achieving consensus in round 3 will provide expert recommendations of the key exercise and dosage variables in the management of chronic non-specific neck pain.Ethical approval was provided by the University of Birmingham Ethics Committee (Ref:ERN_19-1857). Results will be disseminated through peer-reviewed publications and conference presentations.