Clinical guidelines and systematic reviews recommend exercise in the management of chronic non-specific neck pain. Although exercise training programmes that consist of both motor control exercise and exercises for the superficial cervical muscles (segmental exercises) are effective, the exercise variables including dosage vary considerably across trials or are poorly reported.
This study aims to gain expert consensus on these exercise variables so that they can be described clearly using intervention reporting checklists to inform clinical practice and future clinical trials.This protocol for an international Delphi study is informed by the Guidance on Conducting and REporting DElphi Studies recommendations and published to ensure quality, rigour and transparency.
The study will consist of three rounds using anonymous online questionnaires. Expert exercise professionals (physiotherapists, strength and conditioning coaches and so on) and academics in neck pain management will be identified through literature searches, peer referral and social media calls for expression of interest. In round 1, participants will answer open-ended questions informed by intervention and exercise reporting checklists. Responses will be analysed thematically by two independent reviewers.
In round 2, participants will rate their level of agreement with statements generated from round 1 and previous clinical trials using a 5-point Likert scale where 1=strongly disagree and 5=strongly agree. In round 3, participants will re-rate their agreement with statements that achieved consensus in round 2. Statements reaching consensus among participants must meet progressively increased a priori criteria at rounds 2 and 3, measured using descriptive statistics: median, IQR and percentage agreement.
Human NOP58 Ribonucleoprotein (NOP58) ELISA Kit |
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abx381850-96tests | Abbexa | 96 tests | 1093.2 EUR |
NOP58 antibody |
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70R-18924 | Fitzgerald | 50 ul | 522 EUR |
Description: Rabbit polyclonal NOP58 antibody |
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NOP58 siRNA |
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20-abx903599 | Abbexa |
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NOP58 Antibody |
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1-CSB-PA896703LA01HU | Cusabio |
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Description: A polyclonal antibody against NOP58. Recognizes NOP58 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 |
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NOP58 Antibody |
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DF13192 | Affbiotech | 200ul | 420 EUR |
NOP58 siRNA |
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20-abx926153 | Abbexa |
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NOP58 siRNA |
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20-abx926154 | Abbexa |
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NOP58 Antibody |
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1-CSB-PA015940GA01HU | Cusabio |
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Description: A polyclonal antibody against NOP58. Recognizes NOP58 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
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NOP58 Rabbit pAb |
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A14129-100ul | Abclonal | 100 ul | 369.6 EUR |
NOP58 Rabbit pAb |
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A14129-200ul | Abclonal | 200 ul | 550.8 EUR |
NOP58 Rabbit pAb |
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A14129-20ul | Abclonal | 20 ul | 219.6 EUR |
NOP58 Rabbit pAb |
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A14129-50ul | Abclonal | 50 ul | 267.6 EUR |
NOP5/NOP58 antibody |
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70R-3412 | Fitzgerald | 50 ug | 560.4 EUR |
Description: Rabbit polyclonal NOP5/NOP58 antibody raised against the middle region of NOP5/NOP58 |
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NOP58 Polyclonal Antibody |
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30604-100ul | SAB | 100ul | 302.4 EUR |
NOP58 Polyclonal Antibody |
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30604-50ul | SAB | 50ul | 224.4 EUR |
NOP58 Polyclonal Antibody |
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28438-100ul | SAB | 100ul | 302.4 EUR |
NOP58 Polyclonal Antibody |
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28438-50ul | SAB | 50ul | 224.4 EUR |
NOP58 Rabbit pAb |
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A4749-100ul | Abclonal | 100 ul | 369.6 EUR |
NOP58 Rabbit pAb |
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A4749-200ul | Abclonal | 200 ul | 550.8 EUR |
NOP58 Rabbit pAb |
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A4749-20ul | Abclonal | 20 ul | 219.6 EUR |
NOP58 Rabbit pAb |
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A4749-50ul | Abclonal | 50 ul | 267.6 EUR |
NOP58 Blocking Peptide |
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DF13192-BP | Affbiotech | 1mg | 234 EUR |
Anti-NOP58 antibody |
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PAab05796 | Lifescience Market | 100 ug | 426 EUR |
anti- NOP58 antibody |
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FNab05796 | FN Test | 100µg | 606.3 EUR |
Description: Antibody raised against NOP58 |
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Anti-NOP58 antibody |
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STJ116064 | St John's Laboratory | 100 µl | 332.4 EUR |
Description: The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs. |
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Anti-NOP58 antibody |
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STJ24780 | St John's Laboratory | 100 µl | 332.4 EUR |
Description: The protein encoded by this gene is a core component of box C/D small nucleolar ribonucleoproteins. Some box C/D small nucleolar RNAs (snoRNAs), such as U3, U8, and U14, are dependent upon the encoded protein for their synthesis. This protein is SUMOylated, which is necessary for high affinity binding to snoRNAs. |
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pDONR223-NOP58 Plasmid |
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PVTB01144-1 | Lifescience Market | 2 ug | 427.2 EUR |
NOP58 Polyclonal Antibody |
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A71472-050 | EpiGentek | 50 ul | 302.5 EUR |
NOP58 Polyclonal Antibody |
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A71472-100 | EpiGentek | 100 ul | 423.5 EUR |
NOP58 Polyclonal Antibody |
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A54768-020 | EpiGentek | 20 ul | Ask for price |
NOP58 Polyclonal Antibody |
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A54768-050 | EpiGentek | 50 ul | Ask for price |
NOP58 Polyclonal Antibody |
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A54768-100 | EpiGentek | 100 ul | 423.5 EUR |
NOP58 Polyclonal Antibody |
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A54768 | EpiGentek |
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NOP58 Polyclonal Antibody |
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A71472 | EpiGentek |
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NOP5/NOP58 Blocking Peptide |
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33R-5398 | Fitzgerald | 100 ug | 216 EUR |
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of NOP5/NOP58 antibody, catalog no. 70R-3412 |
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NOP58 Antibody, HRP conjugated |
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1-CSB-PA896703LB01HU | Cusabio |
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Description: A polyclonal antibody against NOP58. Recognizes NOP58 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
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NOP58 Antibody, FITC conjugated |
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1-CSB-PA896703LC01HU | Cusabio |
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Description: A polyclonal antibody against NOP58. Recognizes NOP58 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
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NOP58 Antibody, Biotin conjugated |
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1-CSB-PA896703LD01HU | Cusabio |
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Description: A polyclonal antibody against NOP58. Recognizes NOP58 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
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NOP58 Polyclonal Conjugated Antibody |
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C28438 | SAB | 100ul | 476.4 EUR |
Human NOP58 shRNA Plasmid |
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20-abx959853 | Abbexa |
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NOP58 Polyclonal Conjugated Antibody |
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C30604 | SAB | 100ul | 476.4 EUR |
Mouse NOP58 shRNA Plasmid |
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20-abx974581 | Abbexa |
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Rat NOP58 shRNA Plasmid |
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20-abx986026 | Abbexa |
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NOP58 ELISA KIT|Human |
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EF001287 | Lifescience Market | 96 Tests | 826.8 EUR |
NOP58 Recombinant Protein (Human) |
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RP021472 | ABM | 100 ug | Ask for price |
NOP58 Recombinant Protein (Rat) |
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RP214229 | ABM | 100 ug | Ask for price |
NOP58 Recombinant Protein (Mouse) |
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RP154577 | ABM | 100 ug | Ask for price |
Human Nucleolar protein 58 (NOP58) |
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1-CSB-EP896703HU | Cusabio |
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Description: Recombinant Human Nucleolar protein 58(NOP58) expressed in E.coli |
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Nop58 ORF Vector (Rat) (pORF) |
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ORF071411 | ABM | 1.0 ug DNA | 607.2 EUR |
NOP58 ORF Vector (Human) (pORF) |
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ORF007158 | ABM | 1.0 ug DNA | 114 EUR |
Nop58 ORF Vector (Mouse) (pORF) |
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ORF051527 | ABM | 1.0 ug DNA | 607.2 EUR |
NOP58 Polyclonal Antibody, Biotin Conjugated |
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A54765-050 | EpiGentek | 50 ul | 302.5 EUR |
NOP58 Polyclonal Antibody, Biotin Conjugated |
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A54765-100 | EpiGentek | 100 ul | 423.5 EUR |
NOP58 Polyclonal Antibody, FITC Conjugated |
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A54766-050 | EpiGentek | 50 ul | 302.5 EUR |
NOP58 Polyclonal Antibody, FITC Conjugated |
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A54766-100 | EpiGentek | 100 ul | 423.5 EUR |
NOP58 Polyclonal Antibody, HRP Conjugated |
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A54767-050 | EpiGentek | 50 ul | 302.5 EUR |
NOP58 Polyclonal Antibody, HRP Conjugated |
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A54767-100 | EpiGentek | 100 ul | 423.5 EUR |
NOP58 Polyclonal Antibody, Biotin Conjugated |
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A54765 | EpiGentek |
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NOP58 Polyclonal Antibody, FITC Conjugated |
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A54766 | EpiGentek |
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NOP58 Polyclonal Antibody, HRP Conjugated |
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A54767 | EpiGentek |
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Nop58 sgRNA CRISPR Lentivector set (Rat) |
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K6841801 | ABM | 3 x 1.0 ug | 406.8 EUR |
NOP58 sgRNA CRISPR Lentivector set (Human) |
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K1441001 | ABM | 3 x 1.0 ug | 406.8 EUR |
Nop58 sgRNA CRISPR Lentivector set (Mouse) |
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K4828601 | ABM | 3 x 1.0 ug | 406.8 EUR |
Human Nucleolar protein 58, NOP58 ELISA KIT |
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ELI-38275h | Lifescience Market | 96 Tests | 988.8 EUR |
Mouse Nucleolar protein 58, Nop58 ELISA KIT |
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ELI-38276m | Lifescience Market | 96 Tests | 1038 EUR |
Nop58 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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K6841802 | ABM | 1.0 ug DNA | 184.8 EUR |
Nop58 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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K6841803 | ABM | 1.0 ug DNA | 184.8 EUR |
Nop58 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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K6841804 | ABM | 1.0 ug DNA | 184.8 EUR |
NOP58 sgRNA CRISPR Lentivector (Human) (Target 1) |
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K1441002 | ABM | 1.0 ug DNA | 184.8 EUR |
NOP58 sgRNA CRISPR Lentivector (Human) (Target 2) |
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K1441003 | ABM | 1.0 ug DNA | 184.8 EUR |
NOP58 sgRNA CRISPR Lentivector (Human) (Target 3) |
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K1441004 | ABM | 1.0 ug DNA | 184.8 EUR |
Nop58 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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K4828602 | ABM | 1.0 ug DNA | 184.8 EUR |
Nop58 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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K4828603 | ABM | 1.0 ug DNA | 184.8 EUR |
Nop58 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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K4828604 | ABM | 1.0 ug DNA | 184.8 EUR |
NOP58 Protein Vector (Human) (pPB-C-His) |
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PV028629 | ABM | 500 ng | 394.8 EUR |
NOP58 Protein Vector (Human) (pPB-N-His) |
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PV028630 | ABM | 500 ng | 394.8 EUR |
NOP58 Protein Vector (Human) (pPM-C-HA) |
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PV028631 | ABM | 500 ng | 394.8 EUR |
NOP58 Protein Vector (Human) (pPM-C-His) |
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PV028632 | ABM | 500 ng | 394.8 EUR |
NOP58 Protein Vector (Mouse) (pPB-C-His) |
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PV206106 | ABM | 500 ng | 723.6 EUR |
NOP58 Protein Vector (Mouse) (pPB-N-His) |
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PV206107 | ABM | 500 ng | 723.6 EUR |
NOP58 Protein Vector (Mouse) (pPM-C-HA) |
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PV206108 | ABM | 500 ng | 723.6 EUR |
NOP58 Protein Vector (Mouse) (pPM-C-His) |
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PV206109 | ABM | 500 ng | 723.6 EUR |
Human Nucleolar protein 58(NOP58)ELISA Kit |
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QY-E03190 | Qayee Biotechnology | 96T | 472.8 EUR |
NOP58 3'UTR GFP Stable Cell Line |
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TU065825 | ABM | 1.0 ml | 1825.2 EUR |
Nop58 3'UTR GFP Stable Cell Line |
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TU264085 | ABM | 1.0 ml | Ask for price |
NOP58 3'UTR Luciferase Stable Cell Line |
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TU015825 | ABM | 1.0 ml | 1825.2 EUR |
Nop58 3'UTR Luciferase Stable Cell Line |
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TU114212 | ABM | 1.0 ml | Ask for price |
Nop58 3'UTR GFP Stable Cell Line |
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TU164212 | ABM | 1.0 ml | Ask for price |
NOP58 Protein Vector (Rat) (pPB-C-His) |
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PV285642 | ABM | 500 ng | 723.6 EUR |
NOP58 Protein Vector (Rat) (pPB-N-His) |
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PV285643 | ABM | 500 ng | 723.6 EUR |
NOP58 Protein Vector (Rat) (pPM-C-HA) |
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PV285644 | ABM | 500 ng | 723.6 EUR |
NOP58 Protein Vector (Rat) (pPM-C-His) |
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PV285645 | ABM | 500 ng | 723.6 EUR |
Nop58 3'UTR Luciferase Stable Cell Line |
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TU214085 | ABM | 1.0 ml | Ask for price |
NOP58 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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LV692263 | ABM | 1.0 ug DNA | 818.4 EUR |
NOP58 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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LV692267 | ABM | 1.0 ug DNA | 818.4 EUR |
NOP58 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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LV692268 | ABM | 1.0 ug DNA | 818.4 EUR |
Recombinant Candida Albicans NOP58 Protein (aa 1-516) |
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VAng-Lsx05762-inquire | Creative Biolabs | inquire | Ask for price |
Description: Candida Albicans (strain SC5314 / ATCC MYA-2876) Nucleolar protein 58, recombinant protein. |
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Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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K6841805 | ABM | 3 x 1.0 ug | 451.2 EUR |
NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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K1441005 | ABM | 3 x 1.0 ug | 451.2 EUR |
Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
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K4828605 | ABM | 3 x 1.0 ug | 451.2 EUR |
NOP58 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV692264 | ABM | 1.0 ug DNA | 818.4 EUR |
NOP58 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV692265 | ABM | 1.0 ug DNA | 888 EUR |
NOP58 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV692266 | ABM | 1.0 ug DNA | 888 EUR |
Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1) |
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K6841806 | ABM | 1.0 ug DNA | 200.4 EUR |
Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2) |
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K6841807 | ABM | 1.0 ug DNA | 200.4 EUR |
Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3) |
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K6841808 | ABM | 1.0 ug DNA | 200.4 EUR |
NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1) |
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K1441006 | ABM | 1.0 ug DNA | 200.4 EUR |
NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2) |
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K1441007 | ABM | 1.0 ug DNA | 200.4 EUR |
NOP58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) |
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K1441008 | ABM | 1.0 ug DNA | 200.4 EUR |
Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1) |
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K4828606 | ABM | 1.0 ug DNA | 200.4 EUR |
Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2) |
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K4828607 | ABM | 1.0 ug DNA | 200.4 EUR |
Nop58 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3) |
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K4828608 | ABM | 1.0 ug DNA | 200.4 EUR |
Inferential statistics will be used to evaluate measures of agreement between participants (Kendall’s coefficient of concordance) and stability between rounds (Wilcoxon rank-sum test). Statements achieving consensus in round 3 will provide expert recommendations of the key exercise and dosage variables in the management of chronic non-specific neck pain.Ethical approval was provided by the University of Birmingham Ethics Committee (Ref:ERN_19-1857). Results will be disseminated through peer-reviewed publications and conference presentations.